How does network size and topology affect mitochondrial function? To probe feedback in this direction, we are applying genetic manipulations to generate cells with altered network size and topology and then assessing the resultant effect on mitochondrial function.

How does mitochondrial function affect network size and topology? We are comparing mechanisms controlling size and topology in respiring and non-respiring cells. We are also testing mutants with predicted roles in mitochondrial functions for network size and topology phenotypes.

The health of mitochondria can be assessed at the single cell level using fluorescent indicators of membrane potential and redox state, and by fluorescent labeling of mtDNA nucleoids, required for respiratory function. We are combining these methods with our Mitograph approach to map mitochondrial function onto mitochondrial network structure. In this way we can quantify the connection between mitochondrial structure and function both locally within a single network and globally between cells with different network properties and extracellular environments.

membrane potential mapped onto a Mitograph