MitoGraph is a fully automated image processing method and software dedicated to calculating the volume of three-dimensional organelles and intracellular structures in live cells. MitoGraph is currently optimized and validated only for quantifying the volume of tubular mitochondrial networks in budding yeast [1]. However, MitoGraph can also be applied to mitochondria in other cell types and possibly other intracellular (or tissue) structures, with proper validation. MitoGraph is continuously being updated and we hope to be able to accurately analyze mitochondrial network topology and, eventually dynamics. Please contact us if you have questions relating to these other applications that go beyond mitochondrial volume.

To download MitoGraph software please click here

If you use MitoGraph, please cite our main reference:

[1] Viana, MP, Lim S, Rafelski, SM. (2015) Quantifying Mitochondrial Content in Living Cells. In E. Paluch (Ed.), Biophysical Methods in Cell Biology 125: 77-93.